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EXP002413

Paper

Co-Polymer Carrier with Dual Advantages of Cartilage-Penetrating and Targeting Improves Delivery and Efficacy of MicroRNA Treatment of Osteoarthritis (2022)

Peptide

CAP (cartilage-targeting peptide)

Sequence: DWRVIIPPRPSA

RNA

miRNA

All experiment fields

Experiment Id EXP002413
Paper Co-Polymer Carrier with Dual Advantages of Cartilage-Penetrating and Targeting Improves Delivery and
Peptide CAP (cartilage-targeting peptide)
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration 0.5 mg/kg r-miR-140 per intra-articular injection
Rna Concentration 0.5 mg/kg per dose
Mixing Ratio CAP-PVAm-PLGA : r-miR-140 = 1 (w/w)
Formulation Format polymeric nanoparticles (CPP-NPs)
Formulation Components CAP-PVAm-PLGA copolymer + r-miR-140
Size Nm 96.00
Zeta Mv 8.80
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model DMM-induced osteoarthritis mouse model
Administration Route intra-articular injection
Output Type disease modification
Output Value 65% reduction in cartilage degeneration; 80% reduction in synovial inflammation
Output Units
Output Notes Improved cartilage integrity, subchondral bone microarchitecture, and OA scores
Toxicity Notes No systemic toxicity; normal AST/ALT/BUN/CREA
Curation Notes