Modularized Gold Nanocarriers for TAT-Mediated Delivery of siRNA (2016)
Sequence: YGRKKRRQRRR
| Experiment Id | EXP002414 |
|---|---|
| Paper | Modularized Gold Nanocarriers for TAT-Mediated Delivery of siRNA |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~15 µM biotin-TAT during assembly |
| Rna Concentration | ~3 µM surface RNA density on HGNs |
| Mixing Ratio | Mixed biotinylated and non-biotinylated RNA strands (typically 1:1) |
| Formulation Format | gold nanocarrier (hollow gold nanoshell, HGN) |
| Formulation Components | HGN + thiolated RNA + streptavidin + biotin-TAT |
| Size Nm | 50.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells; hESC H9 cells |
| Animal Model | |
| Administration Route | cell incubation followed by NIR laser irradiation |
| Output Type | gene knockdown (light-triggered) |
| Output Value | ~83% reduction of RFP expression under optimal streptavidin coverage |
| Output Units | |
| Output Notes | Efficient uptake shown by dark-field microscopy and flow cytometry; siRNA release and functional knockdown require NIR laser-induced endosomal escape |
| Toxicity Notes | No detectable impact on cell viability reported |
| Curation Notes |