Synthesis and Studies on Cell-Penetrating Peptides (2009)
Sequence: Hca-SRYDDSVPRYHAVRIRKEEREIKDEK
antisense oligonucleotide (AS-ODN)
| Experiment Id | EXP002426 |
|---|---|
| Paper | Synthesis and Studies on Cell-Penetrating Peptides |
| Peptide | Peptide 3a |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~17 nmol peptide per 0.5 nmol AS-ODN |
| Rna Concentration | 500 nM |
| Mixing Ratio | charge ratio rCH = 5 |
| Formulation Format | CPP–ODN electrostatic complex |
| Formulation Components | CPP peptide + AS-ODN |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | NIH/3T3 cells expressing EWS-Fli1 |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | gene expression inhibition |
| Output Value | variable depending on peptide |
| Output Units | |
| Output Notes | All peptides tested for AS-ODN delivery; penetratin derivatives showed strongest functional inhibition. |
| Toxicity Notes | Not reported |
| Curation Notes |