Targeting Cytokine Expression in Glial Cells by Cellular Delivery of an NF-κB Decoy (2007)
Sequence: AGYLLG7KINLKALAALAKKIL
| Experiment Id | EXP002433 |
|---|---|
| Paper | Targeting Cytokine Expression in Glial Cells by Cellular Delivery of an NF-κB Decoy |
| Peptide | Transportan-10 (TP10) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~1 µM |
| Rna Concentration | ~1 µM |
| Mixing Ratio | 1:1 via PNA hybridization |
| Formulation Format | CPP–PNA–DNA decoy complex |
| Formulation Components | TP10 peptide + PNA adaptor + NF-κB decoy |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Primary rat glial cells (astrocytes/microglia) |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | NF-κB activity; IL-6 mRNA expression |
| Output Value | ~80% inhibition of NF-κB binding; ~50% reduction of IL-6 mRNA |
| Output Units | |
| Output Notes | Functional intracellular delivery requires TP10–PNA; decoy alone inactive |
| Toxicity Notes | No toxicity reported for TP10 at used concentrations |
| Curation Notes |