Targeting Cytokine Expression in Glial Cells by Cellular Delivery of an NF-κB Decoy (2007)
Sequence: Cys-ACACAAGGA
| Experiment Id | EXP002434 |
|---|---|
| Paper | Targeting Cytokine Expression in Glial Cells by Cellular Delivery of an NF-κB Decoy |
| Peptide | Cys-PNA 9-mer |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~1 µM (as TP10–PNA conjugate) |
| Rna Concentration | ~1 µM |
| Mixing Ratio | 1:1 via PNA–DNA hybridization |
| Formulation Format | PNA–DNA hybrid within CPP complex |
| Formulation Components | PNA adaptor + NF-κB decoy (requires TP10 for uptake) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Primary rat glial cells |
| Animal Model | |
| Administration Route | cell incubation |
| Output Type | NF-κB activity; IL-6 mRNA expression |
| Output Value | No delivery without TP10; functional only as part of TP10–PNA complex |
| Output Units | |
| Output Notes | PNA alone does not mediate uptake; adaptor role only |
| Toxicity Notes | Not reported |
| Curation Notes |