RNase non-sensitive and endocytosis independent siRNA delivery system (2013)
Sequence: NH2-VGAlAvVvWlWlWlWbA-GSG-PKKKRKVCCOOH
| Experiment Id | EXP002440 |
|---|---|
| Paper | RNase non-sensitive and endocytosis independent siRNA delivery system |
| Peptide | CPP-SWCNT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 8 nmol CPP |
| Rna Concentration | 100 nM siRNA |
| Mixing Ratio | CPP:siRNA 4:1; with SWCNT adsorption |
| Formulation Format | nanotube complex |
| Formulation Components | Single-wall carbon nanotubes, CPP |
| Size Nm | 30.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / apoptosis |
| Output Value | ~70% mTOR knockdown, strong apoptosis |
| Output Units | |
| Output Notes | Endocytosis-independent cytosolic delivery demonstrated |
| Toxicity Notes | Low toxicity in vitro |
| Curation Notes |