Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP002441 |
|---|---|
| Paper | Protein Expression Correlates Linearly with mRNA Dose over Up to Five Orders of Magnitude In Vitro a |
| Peptide | Pepfect14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | no |
| Peptide Concentration | <2 µM |
| Rna Concentration | 1–100 ng mRNA/well (in vitro) |
| Mixing Ratio | N/P = 3 |
| Formulation Format | polyplex |
| Formulation Components | Pepfect14 + mRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, SKOV-3, mGEnC |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | Dose-dependent EGFP and luciferase expression in HeLa and SKOV-3 cells |
| Output Units | |
| Output Notes | Strong uptake but limited expression in mGEnC |
| Toxicity Notes | Low toxicity below 2 µM PF14 |
| Curation Notes |