Sequence: WRAP5 (15-aa CPP, sequence not disclosed in this paper)
| Experiment Id | EXP002446 |
|---|---|
| Paper | In Vivo Follow-Up of Gene Inhibition in Solid Tumors Using Peptide-Based Nanoparticles for siRNA Del |
| Peptide | WRAP5 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 20 µg siRNA (intratumoral) |
| Mixing Ratio | R = 20 |
| Formulation Format | peptide-based nanoparticle |
| Formulation Components | WRAP5 + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | 28.80 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | U87 glioblastoma xenograft (subcutaneous and orthotopic, NSG mice) |
| Administration Route | intratumoral |
| Output Type | in vivo gene knockdown |
| Output Value | ~70% Fluc signal reduction (transient) |
| Output Units | |
| Output Notes | Confirmed mRNA degradation by qRT-PCR |
| Toxicity Notes | No toxicity observed |
| Curation Notes |