Carrier PNA for shRNA delivery into cells (2009)
Sequence: PNA(TGGTGCGAA)-EG-RRRRRRRR
| Experiment Id | EXP002450 |
|---|---|
| Paper | Carrier PNA for shRNA delivery into cells |
| Peptide | Carrier-PNA-R8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | no |
| Peptide Concentration | |
| Rna Concentration | 500 nM shRNA |
| Mixing Ratio | Carrier PNA:shRNA = 1:2 |
| Formulation Format | non-covalent hybrid |
| Formulation Components | PNA–R8 + shRNA hybrid |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-AA8-Luc cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~62–78% luciferase silencing |
| Output Units | |
| Output Notes | RNAi confirmed; sequence-specific delivery |
| Toxicity Notes | |
| Curation Notes |