Sequence: KPRRNRKNKKQKQKQQ
| Experiment Id | EXP002451 |
|---|---|
| Paper | Role of nuclear localization signals in the DNA delivery function of Chikungunya virus capsid protei |
| Peptide | NLS1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 5–20 μM |
| Rna Concentration | 1 μg plasmid DNA |
| Mixing Ratio | peptide:DNA (w/w) up to 7:1 |
| Formulation Format | non-covalent complex |
| Formulation Components | NLS1 peptide + plasmid DNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEK293T; Huh7; BHK21; Vero |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | ~12–14% GFP-positive cells |
| Output Units | |
| Output Notes | Functional DNA delivery without mechanistic escape analysis |
| Toxicity Notes | ≥80% cell viability |
| Curation Notes |