Sequence: guanidine-functionalized α-helical polypeptide (polymeric, sequence not disclosed)
| Experiment Id | EXP002453 |
|---|---|
| Paper | Macrophage membrane-reversibly camouflaged nanotherapeutics accelerate fracture healing by fostering |
| Peptide | PG (α-helical polypeptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 1 µg siRNA/mL |
| Mixing Ratio | PG:siRNA ≈10:1 (w/w) |
| Formulation Format | membrane-cloaked nanocomplex |
| Formulation Components | PG/siRNA + catalase + macrophage membrane + sSDF-1α |
| Size Nm | 170.00 |
| Zeta Mv | -3.30 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | mouse MSCs |
| Animal Model | |
| Administration Route | |
| Output Type | gene silencing / osteogenic differentiation |
| Output Value | ≈68% Ckip-1 knockdown; increased Runx2, ALP, OCN |
| Output Units | |
| Output Notes | ROS-triggered membrane shedding enables cytosolic delivery |
| Toxicity Notes | Low cytotoxicity |
| Curation Notes |