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EXP002454

Paper

Macrophage membrane-reversibly camouflaged nanotherapeutics accelerate fracture healing by fostering MSCs recruitment and osteogenic differentiation (2024)

Peptide

PG (α-helical polypeptide)

Sequence: guanidine-functionalized α-helical polypeptide (polymeric, sequence not disclosed)

RNA

siRNA

All experiment fields

Experiment Id EXP002454
Paper Macrophage membrane-reversibly camouflaged nanotherapeutics accelerate fracture healing by fostering
Peptide PG (α-helical polypeptide)
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 1 mg siRNA/kg
Mixing Ratio PG-based NCs
Formulation Format membrane-cloaked nanocomplex
Formulation Components PG/siRNA + catalase + macrophage membrane + sSDF-1α
Size Nm 170.00
Zeta Mv -3.30
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6 femur fracture mouse model
Administration Route intravenous
Output Type bone healing / gene silencing
Output Value Accelerated fracture healing; improved BMD and callus formation
Output Units
Output Notes Robust in vivo delivery and therapeutic effect
Toxicity Notes No major systemic toxicity
Curation Notes