Sequence: CRGDKGPDC-GRKKRRQRRRC
| Experiment Id | EXP002455 |
|---|---|
| Paper | Tumor Cell Lysate-Based Multifunctional Nanoparticles Facilitate Enhanced mRNA Delivery and Immune S |
| Peptide | TAT-iRGD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 1 µg mRNA |
| Mixing Ratio | MLSV:mRNA = 20:1 (w/w) |
| Formulation Format | lipid–polymer nanoparticle |
| Formulation Components | DOTAP + mPEG-PCL + tumor cell lysate + TAT-iRGD |
| Size Nm | 190.00 |
| Zeta Mv | 47.80 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | B16 melanoma; HEK293T |
| Animal Model | |
| Administration Route | |
| Output Type | protein expression / apoptosis |
| Output Value | High EGFP/Bim expression; apoptosis induction (~87% growth inhibition) |
| Output Units | |
| Output Notes | Caveolin-mediated uptake; efficient cytosolic mRNA translation |
| Toxicity Notes | Low cytotoxicity vs PEI |
| Curation Notes |