Sequence: KKALLAHALHLLAL-LALHLAHALKKA
| Experiment Id | EXP002467 |
|---|---|
| Paper | Comparison of Physicochemical Properties of LipoParticles as mRNA Carrier Prepared by Automated Micr |
| Peptide | LAH4-L1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 400 µg/mL (1/20 w/w) or 100 µg/mL (1/5 w/w) |
| Rna Concentration | 90 ng mRNA per well (DC2.4) |
| Mixing Ratio | mRNA/LAH4-L1 = 1/20 or 1/5 (w/w) |
| Formulation Format | pLbL LipoParticles (microfluidic LPauto) |
| Formulation Components | PLA nanoparticles + DSPC/DOTAP lipid shell (microfluidic) + mRNA + LAH4-L1 |
| Size Nm | 225.00 |
| Zeta Mv | 16.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | DC2.4 murine dendritic cells |
| Animal Model | |
| Administration Route | |
| Output Type | mRNA translation (luciferase assay) |
| Output Value | Efficient expression at 1/20 ratio; reduced expression at 1/5 ratio |
| Output Units | |
| Output Notes | Lower lipid content limits mRNA adsorption and expression at reduced peptide loading |
| Toxicity Notes | High toxicity at 1/20 ratio; improved viability at 1/5 ratio (~75%) |
| Curation Notes |