Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System (2019)
Sequence: R6
| Experiment Id | EXP002469 |
|---|---|
| Paper | Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System |
| Peptide | V2 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Stoichiometric assembly |
| Rna Concentration | 1 µM |
| Mixing Ratio | V1/V1N:siRNA 18:1; V2/V2N:siRNA 9:1 |
| Formulation Format | Collagen–CPP quaternary nanocomplex (~10 nm) |
| Formulation Components | Hybrid peptide subunits + siRNA |
| Size Nm | 10.40 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | NIH/3T3; 3T3-GFP |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake and GFP knockdown |
| Output Value | ~56–58% GFP knockdown for functional complexes |
| Output Units | |
| Output Notes | V1/V1N show siRNA dissociation (low Pearson); V2/V2N remain colocalized (high Pearson) |
| Toxicity Notes | Non-toxic; non-immunostimulatory |
| Curation Notes |