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EXP002472

Paper

Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrangements (2018)

Peptide

KALA

Sequence: WEAKLAKALAKALAKHLAKALAKALKACEA

RNA

siRNA

All experiment fields

Experiment Id EXP002472
Paper Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrange
Peptide KALA
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration Defined by N/P ratio
Rna Concentration 100 pmol per well
Mixing Ratio siRNA:KALA = 1:12–1:24
Formulation Format siRNA/peptide nanocomplex
Formulation Components CTGF siRNA + KALA
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Primary human dermal fibroblasts
Animal Model
Administration Route
Output Type Protein knockdown (Western blot)
Output Value CTGF reduced to ~40–60% of control (p<0.01)
Output Units
Output Notes Significant silencing at 1:12 and 1:24 ratios
Toxicity Notes No major cytotoxicity reported
Curation Notes