Back to browse

EXP002473

Paper

Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrangements (2018)

Peptide

MPG-DNLS

Sequence: GALFLGFLGAAGSTMGAWSQPKSKRKV

RNA

siRNA

All experiment fields

Experiment Id EXP002473
Paper Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrange
Peptide MPG-DNLS
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration Defined by N/P ratio
Rna Concentration 100 pmol per well
Mixing Ratio siRNA:MPGDNLS = 1:1–1:24
Formulation Format siRNA/peptide nanocomplex
Formulation Components CTGF siRNA + MPGDNLS
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Primary human dermal fibroblasts
Animal Model
Administration Route
Output Type Protein knockdown (Western blot)
Output Value No statistically significant CTGF downregulation
Output Units
Output Notes Authors suggest poor endosomal escape / siRNA release
Toxicity Notes Not specifically discussed
Curation Notes