Sequence: LLRLLRWWRLRLLRLLRLL
| Experiment Id | EXP002481 |
|---|---|
| Paper | Enhancing WRAP-Based Nanoparticles for Small Interfering Ribonucleic Acid Delivery in pH-Sensitive E |
| Peptide | P-HyOMe-W5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Defined by molar ratio |
| Rna Concentration | 40–100 nM |
| Mixing Ratio | Peptide:siRNA = 20 |
| Formulation Format | WRAP-based peptide nanoparticles |
| Formulation Components | WRAP5 variant + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | 6.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | EA.hy926 endothelial cells; hiPSC-derived cardiomyocytes |
| Animal Model | |
| Administration Route | |
| Output Type | Protein knockdown (Western blot) |
| Output Value | Up to ~70% FADD knockdown (system- and pH-dependent) |
| Output Units | |
| Output Notes | Cytosolic delivery linked to PEG shedding and restored WRAP5 membrane activity at acidic pH |
| Toxicity Notes | Low cytotoxicity at effective doses |
| Curation Notes |