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EXP002482

Paper

Novel Efficient Cell-Penetrating Peptide–Mediated Strategy for Enhancing Telomerase Inhibitor Oligonucleotides (2015)

Peptide

PepFect15 (PF15)

Sequence: Stearyl-AGYLLG(Ka)LLOOLAAAALOOLL-NH2

RNA

antisense oligonucleotide (LNA/2′OMe mixmer)

All experiment fields

Experiment Id EXP002482
Paper Novel Efficient Cell-Penetrating Peptide–Mediated Strategy for Enhancing Telomerase Inhibitor Oligon
Peptide PepFect15 (PF15)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration Defined by molar ratio
Rna Concentration 1–100 nM
Mixing Ratio PF15:ON = 9:1 (molar)
Formulation Format CPP/oligonucleotide nanocomplex
Formulation Components PF15 + telomerase inhibitor ON1
Size Nm 400.00
Zeta Mv -27.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa cells
Animal Model
Administration Route
Output Type Functional enzyme inhibition (TRAP assay)
Output Value IC50 ≈ 8.4 nM telomerase inhibition
Output Units
Output Notes Strong, dose-dependent telomerase inhibition; >20× potency vs Imetelstat
Toxicity Notes No toxicity reported at effective concentrations
Curation Notes