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EXP002484

Paper

Self-assembled redox-responsive BRD4 siRNA nanoparticles: formulation and its in vitro delivery in gastric cancer cells (2025)

Peptide

LMWP

Sequence: VSRRRRRRGGRRRR

RNA

siRNA

All experiment fields

Experiment Id EXP002484
Paper Self-assembled redox-responsive BRD4 siRNA nanoparticles: formulation and its in vitro delivery in g
Peptide LMWP
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration Defined by N/P ratio
Rna Concentration 40–100 nM
Mixing Ratio N/P = 30
Formulation Format Polymer/CPP siRNA nanoparticles (L-NPs/siBRD4)
Formulation Components LMWP–PEG–SS–PEI + BRD4 siRNA
Size Nm 95.20
Zeta Mv 37.80
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HGC-27; SGC-7901 (gastric cancer cells)
Animal Model
Administration Route
Output Type Gene silencing; proliferation, migration, invasion assays
Output Value Significant BRD4 knockdown; reduced proliferation, migration, invasion
Output Units
Output Notes Enhanced uptake vs PEG-SS-PEI alone; lysosomal escape observed after 6 h
Toxicity Notes Low cytotoxicity at effective doses
Curation Notes