Sequence: GRKKRRQRRR
| Experiment Id | EXP002485 |
|---|---|
| Paper | Studies on the Effect of Lipofectamine and Cell-Penetrating Peptide on the Properties of 10-23 DNAzy |
| Peptide | Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | Varied by N/P ratio |
| Rna Concentration | DZ01 ~150 nM; substrate 1.48 nM |
| Mixing Ratio | N/P ratios from 0.34 to 5.44 |
| Formulation Format | CPP–DNAzyme electrostatic complex |
| Formulation Components | Tat peptide + 10-23 DNAzyme |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro (biophysical/enzymatic) |
| Cell Lines Or Primary Cells | |
| Animal Model | |
| Administration Route | |
| Output Type | Catalytic activity, duplex stability (CD, Tm, PAGE) |
| Output Value | Tat decreases DNAzyme catalytic rate and duplex stability in a charge-dependent manner |
| Output Units | |
| Output Notes | Study evaluates interference of CPP with DNAzyme folding; no cellular delivery or uptake experiments performed |
| Toxicity Notes | Not applicable |
| Curation Notes |