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EXP002494

Paper

Therapeutic uses of peptide nucleic acids (PNA) in oncology (2003)

Peptide

pAntennapedia

Sequence: RQIKIWFQNRRMKWKK

RNA

PNA (antisense)

All experiment fields

Experiment Id EXP002494
Paper Therapeutic uses of peptide nucleic acids (PNA) in oncology
Peptide pAntennapedia
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration Micromolar range
Rna Concentration Micromolar range
Mixing Ratio 1:1 covalent conjugate
Formulation Format CPP–PNA conjugate
Formulation Components pAntennapedia–PNA (disulfide-linked)
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Human melanoma cells
Animal Model
Administration Route
Output Type Telomerase activity (TRAP assay)
Output Value Dose- and time-dependent telomerase inhibition in intact cells
Output Units
Output Notes Fluorescence microscopy confirms intracellular uptake; functional inhibition requires CPP conjugation
Toxicity Notes Mild growth retardation; some apoptosis upon prolonged exposure
Curation Notes