Enhancing RNAi efficiency in Phytophthora infestans using cell-penetrating peptide TAT (2026)
Sequence: AGYLLGKINLKALAALAKKIL
| Experiment Id | EXP002499 |
|---|---|
| Paper | Enhancing RNAi efficiency in Phytophthora infestans using cell-penetrating peptide TAT |
| Peptide | FAM-Tp10 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.2–8 µM |
| Rna Concentration | 200–450 ng per treatment |
| Mixing Ratio | N/P ≈ 2 (TAT/dsRNA only) |
| Formulation Format | CPP/dsRNA electrostatic complex |
| Formulation Components | FAM-Tp10 + dsRNA |
| Size Nm | 1400.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro (plant pathogen) |
| Cell Lines Or Primary Cells | Phytophthora infestans sporangia and hyphae |
| Animal Model | |
| Administration Route | |
| Output Type | RNAi efficiency (qPCR) and uptake imaging |
| Output Value | ≈40% Pigpb1 knockdown for TAT/dsRNA only |
| Output Units | |
| Output Notes | Only TAT enabled functional dsRNA-induced RNAi; others showed uptake only |
| Toxicity Notes | No cell wall damage observed (SEM) |
| Curation Notes |