Multiprotein Silencing Using WRAP-Based Nanoparticles: A Proof of Concept (2025)
Sequence: LLRLLRWWWRLLRLL
| Experiment Id | EXP002509 |
|---|---|
| Paper | Multiprotein Silencing Using WRAP-Based Nanoparticles: A Proof of Concept |
| Peptide | WRAP5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Defined by molar ratio |
| Rna Concentration | Final siRNA 500 nM (per Table 1 conditions) |
| Mixing Ratio | WRAP5:siRNA molar ratio R = 20 |
| Formulation Format | WRAP5/siRNA nanoparticles |
| Formulation Components | WRAP5:siMIX(14/3/3) |
| Size Nm | 79.30 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 glioblastoma; GIST cells (GI stromal tumor) |
| Animal Model | |
| Administration Route | |
| Output Type | Protein knockdown (Western blot) |
| Output Value | Dose-dependent silencing; >80% for optimized conditions (system-dependent) |
| Output Units | |
| Output Notes | Single and mixed siRNA nanoparticles evaluated; mixture enables simultaneous multi-protein silencing |
| Toxicity Notes | Cytotoxicity evaluated; depends on cell density and siRNA dose |
| Curation Notes |