Sequence: RRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP
| Experiment Id | EXP002512 |
|---|---|
| Paper | Suppression of tumor invasion and migration in breast cancer cells following delivery of siRNA again |
| Peptide | PR39 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Up to 36 µM (optimal ~9–18 µM) |
| Rna Concentration | 100 nM |
| Mixing Ratio | siRNA:PR39 = 1:90 |
| Formulation Format | CPP/siRNA noncovalent complex |
| Formulation Components | PR39 + Stat3 siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | 4T1 mouse breast cancer cells |
| Animal Model | |
| Administration Route | |
| Output Type | Gene knockdown and functional invasion/migration assays |
| Output Value | ~50% Stat3 knockdown; strong inhibition of invasion/migration |
| Output Units | |
| Output Notes | PR39 mediates cytoplasmic delivery; synergistic effect with siRNA |
| Toxicity Notes | No cytotoxicity at effective doses |
| Curation Notes |