Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer (2011)
Sequence: ACTGSTQHQCG
| Experiment Id | EXP002520 |
|---|---|
| Paper | Delivery of siRNA and other macromolecules into skin and cells using a peptide enhancer |
| Peptide | SPACE |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 mg/mL (peptide-siRNA conjugate) |
| Rna Concentration | 1 µM |
| Mixing Ratio | Covalent conjugate |
| Formulation Format | Peptide–siRNA conjugate |
| Formulation Components | SPACE–siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | GFP-expressing endothelial cells |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter gene knockdown |
| Output Value | Significant GFP knockdown |
| Output Units | |
| Output Notes | No knockdown with siRNA alone or control peptide |
| Toxicity Notes | No significant cytotoxicity |
| Curation Notes |