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EXP002523

Paper

Targeted tumor-penetrating siRNA nanocomplexes for credentialing the ovarian cancer oncogene ID4 (2012)

Peptide

TP–LyP-1

Sequence: Myristoyl-Transportan-GGGG-LyP-1 (CGNKRTRGC)

RNA

siRNA

All experiment fields

Experiment Id EXP002523
Paper Targeted tumor-penetrating siRNA nanocomplexes for credentialing the ovarian cancer oncogene ID4
Peptide TP–LyP-1
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration Defined by molar ratio
Rna Concentration 25–100 nM
Mixing Ratio siRNA:peptide = 1:20
Formulation Format Tumor-penetrating nanocomplex (TPN)
Formulation Components TP–LyP-1 + siID4
Size Nm 80.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells OVCAR-4; OVCAR-8
Animal Model
Administration Route
Output Type Gene knockdown and apoptosis
Output Value Strong ID4 knockdown; reduced viability
Output Units
Output Notes pH-dependent unpackaging and functional silencing
Toxicity Notes No significant toxicity
Curation Notes