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EXP002530

Paper

Brain tumor-targeted therapy by systemic delivery of siRNA with transferrin receptor-mediated core-shell nanoparticles (2016)

Peptide

T7

Sequence: HAIYPRH

RNA

siRNA

All experiment fields

Experiment Id EXP002530
Paper Brain tumor-targeted therapy by systemic delivery of siRNA with transferrin receptor-mediated core-s
Peptide T7
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration Peptide density 5 mol% of total lipids
Rna Concentration 100 nM
Mixing Ratio Layer-by-layer assembly (core–shell)
Formulation Format T7-modified core–shell nanoparticle (T7-LPC/siRNA)
Formulation Components T7-PEG-DSPE + liposome + protamine/CS/siRNA core
Size Nm 84.00
Zeta Mv 34.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells BMVECs; U87 glioma cells
Animal Model
Administration Route
Output Type Gene knockdown and BBB penetration (co-culture)
Output Value Strong EGFR mRNA and protein reduction
Output Units
Output Notes TfR-mediated uptake enhanced vs PEG control
Toxicity Notes Reduced toxicity after PEGylation
Curation Notes