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EXP002532

Paper

Lipid envelope-type nanoparticle incorporating a multifunctional peptide for systemic siRNA delivery to the pulmonary endothelium (2013)

Peptide

GALA

Sequence: WEAALAEALAEALAEHLAEALAEALEALAA

RNA

siRNA

All experiment fields

Experiment Id EXP002532
Paper Lipid envelope-type nanoparticle incorporating a multifunctional peptide for systemic siRNA delivery
Peptide GALA
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration 2 mol% of total lipid
Rna Concentration Varied (0.2–4 mg/kg equiv in vivo)
Mixing Ratio siRNA/PEI core, lipid encapsulation
Formulation Format GALA-modified MEND
Formulation Components siRNA/PEI core + DOTMA/EPC/Chol + Chol-GALA
Size Nm 100.00
Zeta Mv 15.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HMVEC-L; HUVEC
Animal Model
Administration Route
Output Type Uptake and gene knockdown
Output Value Significant CD31 knockdown
Output Units
Output Notes pH-dependent fusogenic peptide
Toxicity Notes Low toxicity
Curation Notes