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EXP002533

Paper

Lipid envelope-type nanoparticle incorporating a multifunctional peptide for systemic siRNA delivery to the pulmonary endothelium (2013)

Peptide

GALA

Sequence: WEAALAEALAEALAEHLAEALAEALEALAA

RNA

siRNA

All experiment fields

Experiment Id EXP002533
Paper Lipid envelope-type nanoparticle incorporating a multifunctional peptide for systemic siRNA delivery
Peptide GALA
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 2 mol% of total lipid
Rna Concentration 0.5–4.0 mg/kg
Mixing Ratio siRNA/PEI core, lipid encapsulation
Formulation Format GALA-modified MEND
Formulation Components siRNA/PEI core + DOTMA/EPC/Chol + Chol-GALA
Size Nm 100.00
Zeta Mv 15.00
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6 mice; B16F10 lung metastasis model
Administration Route Intravenous
Output Type Tumor suppression and gene knockdown
Output Value ~80% CD31 knockdown; ~50% metastasis reduction
Output Units
Output Notes Efficient lung endothelial targeting
Toxicity Notes No significant toxicity
Curation Notes