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EXP002534

Paper

Development of an antisense RNA delivery system using conjugates of the MS2 bacteriophage capsids and HIV-1 TAT cell penetrating peptide (2009)

Peptide

HIV-1 Tat(47–57)

Sequence: YGRKKRRQRRR

RNA

antisense RNA

All experiment fields

Experiment Id EXP002534
Paper Development of an antisense RNA delivery system using conjugates of the MS2 bacteriophage capsids an
Peptide HIV-1 Tat(47–57)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 100–500 nM (VLP equivalent)
Rna Concentration Encapsulated
Mixing Ratio Covalent conjugation
Formulation Format Tat–MS2 virus-like particle
Formulation Components MS2 VLP + antisense RNA + Tat peptide
Size Nm 26.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Huh-7 cells (HCV luciferase reporter)
Animal Model
Administration Route
Output Type Reporter gene inhibition
Output Value Dose-dependent inhibition of HCV translation
Output Units
Output Notes Incomplete antisense release discussed
Toxicity Notes Higher dose showed toxicity
Curation Notes