Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles (2019)
Sequence: IWLTALKFLGKHAAKHEAKQQLSKL
| Experiment Id | EXP002536 |
|---|---|
| Paper | Delivery of siRNA therapeutics using cowpea chlorotic mottle virus-like particles |
| Peptide | L17E |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~30 peptides per CCMV particle |
| Rna Concentration | ~10 nM |
| Mixing Ratio | ~6:1 (w/w) RNA:CCMV |
| Formulation Format | CPP-modified virus-like particle |
| Formulation Components | CCMV–L17E + siRNA |
| Size Nm | 30.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7 breast cancer cells |
| Animal Model | |
| Administration Route | |
| Output Type | Gene knockdown |
| Output Value | ~50% FOXA1 mRNA reduction |
| Output Units | |
| Output Notes | CCMV alone ineffective; L17E restored silencing |
| Toxicity Notes | No significant toxicity |
| Curation Notes |