Sequence: YGRKKRRQRRR
molecular beacon (antisense probe)
| Experiment Id | EXP002538 |
|---|---|
| Paper | Visualizing the dynamics of viral replication in living cells via Tat peptide delivery of nuclease-r |
| Peptide | Tat(47-57) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.5–2 μM |
| Rna Concentration | 0.5–2 μM |
| Mixing Ratio | 1:1 covalent conjugate |
| Formulation Format | Tat–RNA conjugate |
| Formulation Components | Tat peptide + nuclease-resistant molecular beacon |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Buffalo green monkey kidney (BGMK) cells |
| Animal Model | |
| Administration Route | |
| Output Type | Intracellular viral RNA detection |
| Output Value | Real-time fluorescence upon CVB6 replication; detection of single PFU within 2 h |
| Output Units | |
| Output Notes | Imaging/detection study; no therapeutic knockdown |
| Toxicity Notes | No acute toxicity reported |
| Curation Notes |