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EXP002539

Paper

Hyaluronidase-triggered anticancer drug and siRNA delivery from cascaded targeting nanoparticles for drug-resistant breast cancer therapy (2017)

Peptide

PEGA-pVEC

Sequence: CPGPEGAGCLLIILRRRIRKQAHAHSK

RNA

siRNA

All experiment fields

Experiment Id EXP002539
Paper Hyaluronidase-triggered anticancer drug and siRNA delivery from cascaded targeting nanoparticles for
Peptide PEGA-pVEC
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration ~10 wt% relative to nanoparticles
Rna Concentration ~0.1 µg siRNA per formulation
Mixing Ratio Layer-by-layer assembly
Formulation Format Cascaded targeting nanoparticle
Formulation Components rmSiO2 + siRNA + Dox + HA + PEGA-pVEC
Size Nm 190.00
Zeta Mv -18.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells MDA-MB-231 (CTGF overexpressing breast cancer cells)
Animal Model
Administration Route
Output Type Gene knockdown and apoptosis
Output Value Strong Bcl-xL knockdown; enhanced apoptosis
Output Units
Output Notes siRNA restored chemosensitivity
Toxicity Notes Low toxicity in control cells
Curation Notes