Sequence: GRKKRRQRRRG
| Experiment Id | EXP002543 |
|---|---|
| Paper | Therapeutic Effects in a Transient Middle Cerebral Artery Occlusion Rat Model by Nose-To-Brain Deliv |
| Peptide | Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P ratio 30 |
| Rna Concentration | 1 µg/well (uptake); 0.2 µg/200 µL (toxicity) |
| Mixing Ratio | N/P 5–30 (30 used for main studies) |
| Formulation Format | Polymer micelles (PEG-PCL-Tat) |
| Formulation Components | PEG-PCL-Tat polymer complexed electrostatically with siTNF-α |
| Size Nm | 50.00 |
| Zeta Mv | 19.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | RN33B rat neuronal cells |
| Animal Model | |
| Administration Route | |
| Output Type | Cellular uptake (%) |
| Output Value | Highest uptake at N/P 30; significantly higher than naked siRNA |
| Output Units | |
| Output Notes | Flow cytometry with FAM-siRNA |
| Toxicity Notes | Low cytotoxicity in RN33B cells |
| Curation Notes |