Sequence: GRKKRRQRRRG
| Experiment Id | EXP002544 |
|---|---|
| Paper | Therapeutic Effects in a Transient Middle Cerebral Artery Occlusion Rat Model by Nose-To-Brain Deliv |
| Peptide | Tat |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | PEG-PCL-Tat micelles (N/P 30) |
| Rna Concentration | 30 µg siTNF-α per rat |
| Mixing Ratio | N/P 30 |
| Formulation Format | Polymer micelles (PEG-PCL-Tat) |
| Formulation Components | PEG-PCL-Tat polymer complexed electrostatically with siTNF-α |
| Size Nm | 50.00 |
| Zeta Mv | 19.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Sprague–Dawley rat transient MCAO model |
| Administration Route | Intranasal |
| Output Type | Infarct size, TNF-α level, neurological score |
| Output Value | Significant infarct reduction, TNF-α suppression, neurological improvement |
| Output Units | |
| Output Notes | TTC staining, ELISA, neurological scoring at 22 h post-reperfusion |
| Toxicity Notes | No therapeutic effect from carrier alone |
| Curation Notes |