Sequence: RLWMRWYSPRTRAYGC
| Experiment Id | EXP002545 |
|---|---|
| Paper | Selective Cell Penetrating Peptide-Functionalized Envelope-Type Chimeric Lipopepsomes Boost Systemic |
| Peptide | CPP33 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 20 mol% CPP33 on CLP surface |
| Rna Concentration | 200–400 nM |
| Mixing Ratio | siRNA electrostatically complexed with PLL lumen |
| Formulation Format | Envelope-type chimeric lipopepsomes (CLP) |
| Formulation Components | PEG-b-PAPA-b-PLL (80 mol%) + CPP33-PEG-b-PAPA (20 mol%) |
| Size Nm | 90.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 human lung cancer cells |
| Animal Model | |
| Administration Route | |
| Output Type | PLK1 mRNA and protein knockdown |
| Output Value | Significant sequence-specific PLK1 silencing vs controls |
| Output Units | |
| Output Notes | qRT-PCR + Western blot; confocal shows cytosolic siRNA and reduced lysosomal colocalization |
| Toxicity Notes | Low cytotoxicity in vitro |
| Curation Notes |