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EXP002546

Paper

Selective Cell Penetrating Peptide-Functionalized Envelope-Type Chimeric Lipopepsomes Boost Systemic RNAi Therapy for Lung Tumors (2019)

Peptide

CPP33

Sequence: RLWMRWYSPRTRAYGC

RNA

siRNA

All experiment fields

Experiment Id EXP002546
Paper Selective Cell Penetrating Peptide-Functionalized Envelope-Type Chimeric Lipopepsomes Boost Systemic
Peptide CPP33
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 20 mol% CPP33 on CLP surface
Rna Concentration 150 nmol siPLK1 equiv. kg−1 (q2d ×4)
Mixing Ratio siRNA encapsulated via PLL lumen complexation
Formulation Format Envelope-type chimeric lipopepsomes (CLP)
Formulation Components PEG-b-PAPA-b-PLL (80 mol%) + CPP33-PEG-b-PAPA (20 mol%)
Size Nm 90.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Orthotopic A549-Luc human lung tumor xenograft in nude mice
Administration Route Intravenous (tail vein)
Output Type Tumor growth, survival, biodistribution
Output Value Significant tumor suppression; median survival 45 days vs 26–36 days controls
Output Units
Output Notes Bioluminescence imaging, survival curves, biodistribution
Toxicity Notes Negligible body weight loss; low systemic toxicity
Curation Notes