Sequence: YGRKKRRQRRRYGRKKRRQRRRYGRKKRRQRRR
| Experiment Id | EXP002547 |
|---|---|
| Paper | Gene Silencing Mediated by siRNA-binding Fusion Proteins Is Attenuated by Double-stranded RNA-bindin |
| Peptide | PTD-DRBD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 0.4–3.0 µM protein |
| Rna Concentration | 50 nM siRNA |
| Mixing Ratio | 20–60:1 protein:siRNA |
| Formulation Format | Protein–siRNA complex |
| Formulation Components | PTD-DRBD fusion protein electrostatically complexed with siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | HPRT mRNA knockdown (RT-qPCR) |
| Output Value | ~82% HPRT knockdown (no chloroquine) |
| Output Units | |
| Output Notes | Efficient knockdown; aggregation-mediated uptake; punctate vesicular localization |
| Toxicity Notes | |
| Curation Notes |