Sequence: GHKVKRPKGGHKVKRPKGGHKVKRPKG
| Experiment Id | EXP002549 |
|---|---|
| Paper | Gene Silencing Mediated by siRNA-binding Fusion Proteins Is Attenuated by Double-stranded RNA-bindin |
| Peptide | B2-2×DRBD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 0.4–3.0 µM protein |
| Rna Concentration | 50 nM siRNA |
| Mixing Ratio | 20–60:1 protein:siRNA |
| Formulation Format | Protein–siRNA complex |
| Formulation Components | B2-2×DRBD fusion protein with siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | HPRT mRNA knockdown (RT-qPCR) |
| Output Value | ~30% HPRT knockdown (no chloroquine); ~65% with chloroquine |
| Output Units | |
| Output Notes | Partial receptor-mediated uptake; endosomal trapping dominant |
| Toxicity Notes | |
| Curation Notes |