Sequence: VSRRRRGGRRRRRR
| Experiment Id | EXP002550 |
|---|---|
| Paper | Cellular uptake mechanism and comparative in vitro cytotoxicity studies of monomeric LMWP-siRNA conj |
| Peptide | LMWP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.45–22.5 µM (tested range) |
| Rna Concentration | 0.45–22.5 µM |
| Mixing Ratio | 1:1 LMWP:PEG:siRNA (covalent conjugate) |
| Formulation Format | Covalent CPP–siRNA conjugate |
| Formulation Components | LMWP–PEG–S–S–siRNA (monomeric) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231; MDA-MB-231-EGFP |
| Animal Model | |
| Administration Route | |
| Output Type | Gene silencing + uptake + cytotoxicity |
| Output Value | Highest EGFP knockdown and uptake vs PEI/Lipo; IC50 ~38 µM |
| Output Units | |
| Output Notes | Direct membrane penetration + clathrin/caveolae-independent endocytosis; cytosolic release via disulfide cleavage |
| Toxicity Notes | Low cytotoxicity vs PEI and Lipofectamine |
| Curation Notes |