Photosensitizing Carrier Proteins for Photoinducible RNA Interference (2011)
Sequence: YGRKKRRQRRR
| Experiment Id | EXP002553 |
|---|---|
| Paper | Photosensitizing Carrier Proteins for Photoinducible RNA Interference |
| Peptide | HIV TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 2 µM carrier protein |
| Rna Concentration | 200 nM |
| Mixing Ratio | 10:1 protein:RNA (molar) |
| Formulation Format | CPP–RBP protein/RNA complex (photoinducible CLIP-RNAi) |
| Formulation Components | TatU1A-Alexa546/shRNA or siRNA complexes |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO; EGFP-CHO |
| Animal Model | |
| Administration Route | |
| Output Type | EGFP gene silencing (light-dependent) |
| Output Value | ≈70–80% EGFP knockdown after photoirradiation |
| Output Units | |
| Output Notes | Endosomal entrapment before light; cytosolic release and RNAi after 540 nm irradiation |
| Toxicity Notes | No detectable phototoxicity under irradiation conditions |
| Curation Notes |