A solid-phase method for peptide–siRNA covalent conjugates based on click chemistry (2012)
Sequence: LALLAK
| Experiment Id | EXP002556 |
|---|---|
| Paper | A solid-phase method for peptide–siRNA covalent conjugates based on click chemistry |
| Peptide | LALLAK |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50 nM |
| Mixing Ratio | Covalent 1:1 peptide:siRNA conjugate |
| Formulation Format | Covalent peptide–siRNA conjugate |
| Formulation Components | LALLAK–siRNA conjugate via 1,2,3-triazole linker |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEK293 |
| Animal Model | |
| Administration Route | |
| Output Type | Gene silencing efficiency (luciferase reporter) |
| Output Value | ≈90–93% knockdown comparable to unmodified siRNA |
| Output Units | |
| Output Notes | Passenger-strand conjugation retains RNAi; guide-strand conjugation slightly reduces efficiency |
| Toxicity Notes | |
| Curation Notes |