GCSP (guanidinylated cyclic synthetic polypeptide)
Sequence: synthetic polypeptide (guanidinylated poly(allyl-L-glutamate) backbone)
| Experiment Id | EXP002561 |
|---|---|
| Paper | Guanidinylated Cyclic Synthetic Polypeptides Can Effectively Deliver siRNA by Mimicking the Biofunct |
| Peptide | GCSP (guanidinylated cyclic synthetic polypeptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | N/P = 5–20 |
| Rna Concentration | 30–60 nM |
| Mixing Ratio | N/P ratio dependent |
| Formulation Format | Polymeric polypeptide–siRNA nanocomplex |
| Formulation Components | GCSP/siRNA electrostatic nanocomplex |
| Size Nm | 80.00 |
| Zeta Mv | 5.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-Luc |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase gene silencing |
| Output Value | Up to ~80% knockdown (CPG98) |
| Output Units | |
| Output Notes | Endocytic inhibitors + 4 °C uptake; LysoTracker escape; nuclear accumulation |
| Toxicity Notes | Comparable or lower than PEI/Lipo2000 |
| Curation Notes |