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EXP002569

Paper

Crotamine/siRNA Nanocomplexes for Functional Downregulation of Syndecan-1 in Renal Proximal Tubular Epithelial Cells (2023)

Peptide

Crotamine

Sequence: YKQCHKKGGHCFPKEKICLPPSSDFGKMDCRWRWKCCKKGSG

RNA

siRNA

All experiment fields

Experiment Id EXP002569
Paper Crotamine/siRNA Nanocomplexes for Functional Downregulation of Syndecan-1 in Renal Proximal Tubular
Peptide Crotamine
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration
Mixing Ratio 50:1 and 100:1 (crotamine:siRNA)
Formulation Format CPP–siRNA electrostatic nanocomplex
Formulation Components Crotamine + siRNA
Size Nm 125.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HK-2 human proximal tubular epithelial cells
Animal Model
Administration Route
Output Type Gene knockdown and complement activation
Output Value Syndecan-1 mRNA/protein ↓ ~50%; reduced properdin binding and C3 activation
Output Units
Output Notes
Toxicity Notes
Curation Notes