SCO

Linked experiment: EXP000833

Linked peptide: Penetratin

Linked paper: Structural Requirements for Cellular Uptake and Antisense Activity of Peptide Nucleic Acids Conjugated with Various Peptides (2006)

RNA overview

RNA Type: SCO
Payload/target: 18-mer PNA targeting IVS2-705 cryptic splice site in β-globin intron 2 inserted into luciferase reporter (splice correction)
Target gene/transcript: luciferase; IVS2-705 cryptic splice site in β-globin intron 2

Sequence information

Sequence reported in source article: yes
RNA sequence: PNAAcC:cccctttacctcagttacaPNAscr:tccttcccaaactttgaca
Sense strand: CCCCTTTACCTCAGTTACA
Antisense strand

Chemical modifications

RNA modifications: PNA with (AEEA/ethylene glycol) spacers (ooo) x3 at both termini; some conjugates FAM-labeled for confocal microscopy