siRNA

Linked experiment: EXP001072

Linked peptide: CADY

Linked paper: HER2 RNA Aptamer- and Cell Penetrating Peptide-Mediated Delivery of Multimeric Antisense Strands of siRNAs for Gene Silencing (2016)

RNA overview

RNA Type: siRNA
Payload/target: eGFP (enhanced GFP) in eGFP-expressing cells
Target gene/transcript: EGFP

Sequence information

Sequence reported in source article: yes
RNA sequence: sense:UGCGCUCCUGGACGUAGCCUU; antisense:TGCGCTCCTGGACGTAGCCTTAGCCGCGCCCTACCCTATCCCTCCCCTCGCGGCTCCCTATAGTGAGTCGTATTAGTCC
Sense strand: UGCGCUCCUGGACGUAGCCUU
Antisense strand: TGCGCTCCTGGACGTAGCCTTAGCCGCGCCCTACCCTATCCCTCCCCTCGCGGCTCCCTATAGTGAGTCGTATTAGTCC

Chemical modifications

RNA modifications: Antisense strand is di-thiolated at 5′ and 3′ ends and polymerized into multimeric antisense strands (>150–500-mer by gel, SI); annealed to 8-mer PNA complementary to antisense