siRNA

Linked experiment: EXP001112

Linked peptide: DEN-K(GALA)-TAT

Linked paper: Design and evaluation of a stearylated multicomponent peptide-siRNA nanocomplex for efficient cellular siRNA delivery (2017)

RNA overview

RNA Type: siRNA
Payload/target: eGFP (reporter) in stable eGFP-expressing HeLa cells
Target gene/transcript: EGFP; HeLa cells

Sequence information

Sequence reported in source article: yes
RNA sequence: sense:GCAGCACGACUUCUUCAAGdTdT; antisense:CUUGAAGAAGUCGUGCUGCdTdT
Sense strand: GCAGCACGACUUCUUCAAGdTdT
Antisense strand: CUUGAAGAAGUCGUGCUGCdTdT

Chemical modifications

RNA modifications: Synthetic eGFP siRNA duplex (sense 5′-GCA GCA CGA CUU CUU CAA G dTdT-3′; antisense 5′-CUU GAA GAA GUC GUG CUG C dTdT-3′). Alexa Fluor 488-labeled sense used for uptake/trafficking assays.