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pDNA

Linked experiment: EXP001320

Linked peptide: KALA

Linked paper: Mitochondrial transgene expression via an artificial mitochondrial DNA vector in cells from a patient with a mitochondrial disease (2018)

RNA overview

RNA Type

pDNA

Payload/target

pCMV-mtLuc (CGG) [hND4] (mitochondrial reporter plasmid encoding NanoLuc with mitochondrial codon adjustments; includes human ND4 sequence)

Target gene/transcript

NanoLuc with mitochondrial codon adjustments

Sequence information

Sequence reported in source article

no

RNA sequence

not reported

Sense strand

Antisense strand

Chemical modifications

RNA modifications

Cy5-labeled pDNA used for uptake/CLSM; protamine-condensed pDNA at N/P 2.3 prior to lipid coating