sgRNA

Linked experiment: EXP001340

Linked peptide: TAT-ABE8e (covalent CPP fusion)

Linked paper: Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations (2025)

RNA overview

RNA Type: sgRNA
Payload/target: rd12-targeting sgRNA (ABE) or epegRNA (PE) for Rpe65 rd12 mutation correction (R44X); non-targeting guides used as controls in some experiments.
Target gene/transcript: guides used as controls in some experiments; rd12-targeting; epe

Sequence information

Sequence reported in source article: yes
RNA sequence: sense:ACATCAGAGGAGACTGCCCAGGTTTTAGAGCTAGAAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
Sense strand: ACATCAGAGGAGACTGCCCAGGTTTTAGAGCTAGAAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
Antisense strand

Chemical modifications

RNA modifications: End-modified guide RNAs: 2′-O-methyl on first 3 and last 3 nucleotides; first 3 and last 3 phosphodiester bonds replaced with phosphorothioates (as described in Methods).